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United States Department of Agriculture

Agricultural Research Service

Title: EFFECT OF MASTECTOMY ON PHENOTYPE AND FUNCTION OF LEUKOCYTES IN PERIPARTURIENT DAIRY COWS)

Author
item Kimura, Kayoko
item Goff, Jesse
item Kehrli jr, Marcus
item Harp, James
item Nonnecke, Brian

Submitted to: International Virtual Conference on Infectious Diseases of Animals
Publication Type: Abstract only
Publication Acceptance Date: 4/20/1997
Publication Date: N/A
Citation:

Interpretive Summary:

Technical Abstract: We have previously established that there is a significant decline in several T-cell subset populations and diminished lymphocyte and neutrophil function in periparturient dairy cows. We hypothesized that milk production may be an immunosuppressive factor. To test this hypothesis, we used 6 mastectomized and 6 intact multiparous Jersey cows (all 6 intact cows developed milk fever). Flow cytometry permitted phenotype analysis of peripheral blood mononuclear cells (PBMC) using monoclonal antibodies against T-cell subsets, B-cells, and monocytes. A leukogram was also done to determine absolute numbers of each cell subset. Lymphocyte blastogenesis (LBT) and polymorphonuclear neutrophil iodination (PMN-IOD) were done to assess functional ability of leukocytes. T-cell populations decreased significantly from 27 d before calving until calving in intact cows (CD3 from 51.3 to 42.7%, CD4 from 29.1 to 25.8%, CD8 from 12.4 to 10.0%, and gd T-cell from 7.6 to 4.8%). In intact cows, monocyte numbers increased at parturition (from 19.3 to 31.6% of the PBMC). In mastectomized cows, only the population of CD3 cells was decreased at parturition (from 49.9 to 45.5%).

Last Modified: 8/24/2016
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