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ARS Home » Research » Publications at this Location » Publication #79730
Title: ENHANCED DISCRIMINATION OF ESCHERICHIA COLI FLAGELLA USING MONOCLONAL ANTIBODIES AND DNA SEQUENCE DATA

Author
item Kwang, Hwei Sing
item Yang, Xiao
item WILSON, RICHARD - PENNSYLVANIA STATE UNIV

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 4/21/1997
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: According to published data, within the group of 54 identifiable E. coli flagellar antigens, the serotypes of H2 and H7, regardless of O type, are among the five most frequent H-types isolated from human EPEC, EHEC and ETEC patients. However, the conventional tube agglutination test using polyclonal antibody to classify H2 and H7 often react with the non-homologous antigen on the standard strains. Specifically, H2 cross-reacts with H7, 12, 23, 28, 38, 44, 51 and 56. H7 cross-reacts with H12, 27, 28, 41, 44, 51 and 56. Surprisingly, the true "H" was discriminated often by conventional wisdom based upon the level of agglutination activities. Identification of the epitopes for each "H" by monoclonal antibody or detection of specific sequences for each "H" and circumvent induction of motility to identify flagella type will allow precise typing of E. coli. Because a limited number of flagella serotypes are associated with pathogenic O groups, ac curate identification is important in diagnostics and epidemiological studies. Therefore, we have developed monoclonal antibodies specific to E. coli H2 and H7 flagella and, more importantly, have identified the minimal sequences which define the sero-specificity for H2 and H7.