Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 4/15/1997
Publication Date: N/A
Technical Abstract: Rhopalosiphum padi virus (R. padi virus) infects Rhopalosiphum padi, one of the aphid vectors of barley yellow dwarf viruses, and has been detected in both oat plants and aphids. Since purified R. padi virus RNA did not replicate in oat protoplasts, it is likely that the virus does not replicate in oat plants, but uses them as a reservoir. The approximately 10 kb RNA genome of R. padi virus, poly(A)-tailed on 3 cents end, was isolated from purified R. padi virus, used to synthesize cDNAs using random and oligo-dT primers, subcloned and partially sequenced. Sequence alignments showed that one subclone had amino acid sequence similarity to the RNA-dependent RNA polymerase of parsnip yellow fleck virus (PYFV) a picona-like virus. Using polyclonal antibodies raised to purified R. padi virus, western blotting analysis showed that R. padi virus also has three coat proteins (approximately 20 kDa, 25 kDa, 30 kDa) as does PYFV.