Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 4/12/1997
Publication Date: N/A
Technical Abstract: Barley yellow dwarf viruses (BYDV) cause the most economically important and widespread viral diseases of cereal grains. Significant levels of tolerance to BYDV infection have been developed in selected oat lines. However, the complex genetics of the trait have made moving the tolerance to new lines difficult. To identify markers linked to loci contributing to BYDV tolerance in oat, near-isogenic lines (NILs) and a recombination inbred (RI) population were developed from the Clintland64 (BYDV-sensitive) and IL86-5698 (BYDV-tolerant) oat lines. Markers for tolerance loci were identified using amplified fragment length polymorphism (AFLP) analysis. DNA samples from three pairs of NILs and the parents were amplified with 80 different combinations of EcoRI and MseI primers. Also, selected combinations of primers were applied to 126 individuals of the RI population. Data on the presence or absence bands were collected. The contributions to BYDV tolerance of loci were estimated using from BYDV field data and segregation of the markers in the RI population. The markers identified by banding patterns that perfectly cosegregated with tolerance in the NILs contributed between 16 and 25% of the tolerance. Those loci with significant contributions to tolerance (P<0.01) were placed on the hexaploid oat RFLP map by analyzing the segregation of the markers in the Kanota x Ogle RI population. Four loci were identified that explained approximately 68% of the variation seen in the Clintland64 x IL86-5698 RI population.