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ARS Home » Southeast Area » Charleston, South Carolina » Vegetable Research » Research » Publications at this Location » Publication #78392
Title: GENETIC MAPPING OF MUSKMELON (CUCUMIS MELO L.) USING AMPLIFIED FRAGMENT LENGTH POLYMORPHISM (AFLP) MARKERS

Author
item WANG, YIHONG - CLEMSON UNIVERSITY
item Thomas, Claude
item DEAN, RALPH - CLEMSON UNIVERSITY

Submitted to: Plant and Animal Genome VX Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 1/12/1997
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Muskmelon (Cucumis melo L.) has 12 chromosomes (2n=2x=24) with a genome size of 5 x 10(8th) bp. A genetic map with sufficient markers is not yet available. For construction of a map with higher density we identified Amplified Fragment Length Polymorphic (AFLP) markers. The mapping population consisted of sixty-six backcross progenies derived from MR-1 (a breeding line with multiple disease resistance) and Ananas Yokneam (AY, an Israeli landrace susceptible to many diseases) with AY as recurrent parents. Only markers present in MR-1 were included in the map. Initial screening of parents identified 426 markers, but only 221 (together with RAPD markers) were scored in the progeny. Ninety percent of the markers fell into 18 linkage groups with 2-27 markers in each group using a lod score of 4 and maximum theta of 0.25. The map covers over 2000 cM. Several markers linked to Fom-2, a gene for resistance to Fusarium wilt were identified.