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ARS Home » Research » Publications at this Location » Publication #78377


item Ronning, Catherine
item Sanford, Lind
item Stommel, John

Submitted to: Journal of American Society of Horticulture Science
Publication Type: Abstract Only
Publication Acceptance Date: 6/1/1997
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Colorado potato beetle (CPB) is a destructive pest of the cultivated potato. Certain glycoalkaloids in potato leaves are effective deterrents to this insect; however, in tubers these compounds can be toxic to humans. Leptines are foliar specific glycoalkaloids produced by the related species, Solanum chacoense. We are studying the inheritance of leptine production in segregating F-1 and F-2 populations derived from two S. chacoense accessions, 55-1 and 55-3, which are (respectively) high and low leptine producers. The F-1 segregates 1:1 for high (>70% of total glycoalkaloids) and low (<20% of TGA) leptine content. Segregation data from the F-1 and F-2 populations suggest a two-gene model for leptine production: a dominant repressor and a recessive inducer. Using two bulked DNA samples composed of high and low-leptine individuals from the F-1 population, we are using various molecular markers (RAPDs, SSRs, DS-PCR, and AFLPs) to search for markers linked to leptine production. We have identified a RAPD band which appears to be closely associated with low leptine content and supports the two-gene model. The use of such a marker in a breeding program will facilitate the development of CPB resistant potato varieties.