Submitted to: BARC Poster Day
Publication Type: Abstract Only
Publication Acceptance Date: 2/1/1997
Publication Date: N/A
Technical Abstract: Colorado potato beetle (CPB) is a destructive pest of the cultivated potato, Solanum tuberosum. Certain glycoalkaloids in potato leaves are effective deterrents to this insect; however, in tubers these compounds can be toxic to humans. Leptines are foliar-specific glycoalkaloids produced by the related species, S. chacoense. These compounds have been shown to confer resistance to CPB. We are studying the inheritance of leptine production in segregating F-1 and F-2 populations derived from two S. chacoense accessions, which are (respectively) high and low leptine producers. The F-1 segregates 1:1 for high (>70% of total glycoalkaloids) and low (<20% of TGA) leptine content. Segregation data from the F-1 and F-2 populations suggest a two-gene model for leptine production. Using two bulked DNA samples composed of high- and low-leptine individuals from the F-1 population, we are screening various molecular markers to find markers linked to leptine production. We have identified a RAPD band which appears to be closely associated with low leptine content and supports the two-gene model. The use of such a marker in a breeding program will facilitate the development of CPB resistant potato varieties.