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ARS Home » Midwest Area » St. Paul, Minnesota » Plant Science Research » Research » Publications at this Location » Publication #76883
Title: MAIZE CHROMOSOME-SPECIFIC LIBRARIES FROM OAT-MAIZE CHROMOSOME ADDITION LINES

Author
item ANANIEV, EVGUENI - UNIVERSITY OF MINNESOTA
item RIERA-LIZARAZU, OSCAR - UNIVERSITY OF MINNESOTA
item PHILLIPS, RONALD - UNIVERSITY OF MINNESOTA
item Rines, Howard

Submitted to: Agronomy Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 12/1/1996
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Maize (Zea mays L.) chromosome addition lines of oat (Avena sativa L.) recovered from oat x maize crosses serve as a means to clone individual maize chromosomes. These lines also provide a unique opportunity to produce by irradiation a series of oat-maize introgression lines with small segments of an individual maize chromosome added to an oat genome. Substantial differences between the oat and maize genomic DNA have enabled development of a highly effective screening procedure for detecting introgressed maize DNA using maize-specific dispersed repetitive DNA sequences. A cosmid library was constructed from DNA of a maize chromosome 9 addition line and screened with the help of a multiprobe composed of a mixture of maize repetitive sequences. A set of 250 maize-specific cosmid clones from maize chromosome 9 was used to determine the distribution of different repeated sequences and to isolate additional repeated sequences as well as a set of unique sequences specific for chromosome 9.