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United States Department of Agriculture

Agricultural Research Service


item Brown, Mark
item Lightner, Gary

Submitted to: Entomophaga
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/1/1997
Publication Date: N/A
Citation: N/A

Interpretive Summary: The size of an experimental plot is important for sampling insect populations. The plot must be large enough so that the samples reflect the true effects of an experiment. This paper is the first to present data which shows how large a plot sampling insect predators in apple orchards needs to be. Based on insect numbers per tree, we recommend that 3 acres are needed to sample insect predators and 3.7 acres are needed to sample spiders. These recommendations will be useful to researchers designing experiments on insect and spider populations in apple orchards.

Technical Abstract: Five apple orchards in West Virginia, USA, were managed with different pest management practices ranging from unsprayed to conventional insecticides. Data were collected on aphid, predaceous insect, and spider abundance every 2 weeks from May to July along one or two randomly selected, 15 consecutive tree transects. A total of 892 individual predaceous insects were observed: 32% were Aphidoletes aphidimyza, 21% were Coccinella septempunctata, and the rest were from 13 other taxa. The most important predator controlling aphid populations was C. septempunctata. Of the 32 sample transects with predators, 72% showed an edge effect in which either the standard error or mean of predator abundance differed from the edge towards the center of the orchard. This edge effect extended a mean of 7 trees (30 m) into the orchard. Only 40% of the 32 transects that had spiders showed an edge effect, extending into the orchard a mean of 8 trees (33m). However, 75% of the transects with more than one spider per tree had an edge effect of 10 trees (43m). We recommend a minimum border of 7 trees before sampling for aphid predators and 10 trees before sampling for spiders. A recommended experimental plot size, for semi-dwarf apple orchards, to eliminate edge effects is 1.25ha for aphidophaga, 1.5ha for spiders; a minimum plot size of 0.6ha for aphidophaga and 0.75ha for spiders would minimize edge effects.

Last Modified: 10/17/2017
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