|Nisbet, David - Dave|
|Byrd Ii, James - Allen|
Submitted to: United States Animal Health Association Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 10/18/1996
Publication Date: N/A
Technical Abstract: We are developing a defined competitive exclusion culture that will increase the resistance of newborn and weaning pigs to gastrointestinal colonization of Salmonella. A 28-day weaned pig that had been maintained on a typical swine ration at the National Animal Disease center in Ames was sacrificed and the cecal contents immediately transferred to an anaerobic chamber and processed by methods previously described resulting in the continuous-flow-derived competitive exclusion culture (CFCE). The litter from three sows were assigned to 3 different experimental groups, non-Salmo challenged negative controls (group 1), Salmonella challenged positive controls (group 2), and a Salmonella challenged CF culture treated litter (group 3). The litter receiving the culture was provided 5 mls of culture ca. 4 h post-farrowing and again at 24 h. Pigs in groups 2 and 3 were challenged with 10**3 S. choleraesuis. Pigs were sacrificed 7 days after challenge and Salmonella gut colonization measured. Because both control groups of pigs were shedding serotype B Salmonella, no data for the S. choleraesuis is give and colonization control data is based upon total salmonellae cultivated. Salmonella-positive rectal swabs were 34/40 (85%); 72/72 (100%); and 10/56 (18%), respectively, for groups 1, 2, and 3. Salmonellae-positive colonic samples were 100%, 67%, and 0% for groups 1, 2, and 3, respectively. Ileal cecal junction salmonellae-positive samples were 80%, 100%, and 14% for groups 1, 2, 3, respectively. Cecum salmonellae-positive samples for groups 1, 2, and 3 were 80%, 100%, and 0%, and salmonellae cecal-positive samples for these groups were 80%, 100%, and 28%, respectively. Total salmonellae cfu in cecal and ileal cecal contents were also decreased in the CF-treated group compared to control groups.