|Smith, Timothy - Tim|
Submitted to: Mammalian Genome
Publication Type: Abstract Only
Publication Acceptance Date: 1/20/1997
Publication Date: N/A
Technical Abstract: Nebulin (NEB) belongs to a family of large molecular weight proteins (600 to 900 kD) present in various skeletal muscles. They constitute a set of inextensible filaments anchored at the Z line that act as a length-regulating template for thin filaments. Nebulin was initially assigned to 2q31-q32 in humans, and more recently to 2q24.1-q24.2 in a synteny group that covers 5-10 cM on human chromosome 2 conserved since th divergence of humans and rodents. Glucagon (GCG), a polypeptide hormone found in the pancreas and guts of mammals, exerts significant effects on gluconeogenesis, glycogenolysis and the release of glucose by hepatocytes. The glucagon gene has been assigned to the long arm of human chromosome 2 (2q23) by isotopic in situ hybridization. In the present study we report a direct comparison of the physical location of glucagon and nebulin in cattle, sheep and goat. The purpose of this study was to test the utility of previous physical assignments in humans located in putatively conserved chromosomal regions between species to expedite the development of gene maps in domestic species.