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ARS Home » Pacific West Area » Aberdeen, Idaho » Small Grains and Potato Germplasm Research » Research » Publications at this Location » Publication #74932

Title: PCR-RAPD VARIATION AMONG HULLESS BARLEY ACCESSIONS

Author
item Hang, An
item Hoffman, David
item Burton, Charlotte
item LIU, CHIA-TSANG

Submitted to: American Society of Agronomy
Publication Type: Abstract Only
Publication Acceptance Date: 11/3/1996
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Knowledge of genetic diversity is important for the improvement of crop species. Thirty-six hulless barley accessions from North America, China, Turkey, and Central Asia were analyzed using the randomly amplified polymorphic DNA (RAPD) technique. Forty-seven major reproducible fragments were produced with nine random decamer primers. Variation in fragment patterns was observed between accessions in all primers studied. Cluster analysis of genetic similarities among accessions revealed that most of the accessions from North America are closely related and belong to one well-defined cluster. Based on their genetic distance, those from China, Turkey, and Central Asia belong to another group that can be divided into three sub-clusters. RAPD markers therefore can be used to estimate genetic distances among barley accessions.