Submitted to: Molecular Markers and Plant Breeding
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/10/1996
Publication Date: N/A
Interpretive Summary: DNA markers are used to create "road maps" of the chromosomes of plant and animal species. The positions of genes controlling important traits such as disease or insect resistance, fruit or grain quality, resistance to environmental stresses, etc. can be established in reference to the genetic markers or "milestones" on the genetic map. When a marker is situated near a gene controlling a trait of interest, the plant breeder can use the marker to select for the trait of interest and thereby avoid the cost and time required to assess the level of disease or insect resistance, fruit or grain quality, etc. A new and versatile type of DNA marker is based on small DNA fragments called Simple Sequence Repeats (SSRs). This report documents the development of a new SSR marker in corn. It also reports the positioning or mapping of this new marker, MZECT34, near the center of chromosome 10 in corn. This SSR marker is one more tool that can be used by corn breeders in their efforts to genetically improve corn.
Technical Abstract: Microsatellites or simple sequence repeats (SSRs) have been described as the second generation of polymorphic DNA markers. A new maize SSR, MZECT34 was developed by screening a Sau3A1 genomic library of inbred line J105 with a (CT)8 oligonucleotide probe. DNA sequence determination indicated that MZECT34 has a (TC)12 core motif. MZECT34 was mapped to maize chromosome 10 in a 77 plant F2 population of the cross of inbreds LI731a x W6786.