|Rimler, Richard - Rick|
Submitted to: Research Workers in Animal Diseases Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 10/11/1996
Publication Date: N/A
Technical Abstract: Pasteurella multocida causes fowl cholera in turkeys. With serogroup A, capsular hyaluronic acid (HA) promotes adhesion to elicited turkey air sac macrophages (TASM). In contrast, freshly isolated turkey peripheral blood monocytes (TPBM) do not bind serogroup A strains. In vitro maturation of TPBM over a period of 6 days resulted in gradual increased adhesion of the bacteria. Coating of glass slides with Entactin-Collagen IV-Laminin attachment (ECL) matrix further enhanced the adhesion of P. multocida. Addition of hyaluronic acid to the TPBM cultures inhibited bacterial adherence similar to that previously observed for TASM. Exposure of TPBM to a MAb directed against HA binding cell surface proteoglycan (CD44) decreased the binding of P. multocida. Collectively, these data indicate that P. multocida adhesion to TPBM is promoted by capsular hyaluronic acid and can be upregulated by maturation on ECL matrix or following hyaluronidase treatment. Also, the data suggest that recognition of the capsular mucopolysaccharide is due to a monocytic CD44 isoform present on cultured TPBM.