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United States Department of Agriculture

Agricultural Research Service

Title: MOLECULAR CHARACTERIZATION OF THINOPYRUM CHROMATIN IN WHEAT: TOWARD TARGETED MAPPING OF BYDV RESISTANCE)

Author
item Crasta, Oswald
item Francki, Michael
item Greene, Ann
item Bucholtz, Dennis
item Sharma, Hari
item Herbert, Ohm
item Anderson, Joseph

Submitted to: International Crop Science Congress Proceedings
Publication Type: Abstract only
Publication Acceptance Date: 7/16/1996
Publication Date: N/A
Citation:

Interpretive Summary:

Technical Abstract: Barley yellow dwarf virus (BYDV) is a devastating disease of cereals worldwide and is the most serious viral disease of wheat. Wheatgrass (Thinopyrum intermedium) is an excellent source of BYDV resistance. Various alien addition and substitution lines (AALs and ASLs) of wheat containing wheatgrass chromatin have been developed and the BYDV-resistance e(BYDV(R)) locus is contained on the introgressed chromosome 7E. The major objective of this research project is to characterize the wheatgrass chromatin introgressed into wheat using molecular and cytogenetic techniques as a first step toward mapping of BYDV(R) locus. Our research includes using fluorescence in situ hybridization (FISH) to identify appropriate deletion and translocation lines and determine the physical size of the introgressed wheatgrass chromatin. The introgression lines are further being characterized using DNA markers, that have been shown to be colinear across different grass species. Nine markers, spanning group 7 chromosome of Triticeae have been used to characterize chromosome 7E and more markers are being identified and these markers will be used to identify breakpoints in deletions and translocations. Two molecular techniques, representational difference analysis and differential display PCR, are being used with resistant translocation and susceptible sister lines to specifically target genomic DNA and cDNA markers associated with the BYDV(R) locus. These results on molecular characterization of wheatgrass chromatin in wheat as a basis for mapping of the BYDV(R) locus will be discussed.

Last Modified: 8/24/2016
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