Submitted to: Meeting Abstract
Publication Type: Proceedings
Publication Acceptance Date: 3/13/1996
Publication Date: N/A
Citation: Interpretive Summary:
Technical Abstract: A defined competitive exclusion culture that enhances colonization resistance against salmonellae in suckling pigs was investigated. Previously in our laboratory it was shown that 10-day-old broiler and layer chicks that were fed diets containing 5-10% lactose provided either in the feed or in water from day of hatch were significantly more resistant to Salmonella typhimurium, and S. enteritidis cecal colonization than control chicks not provided a diet supplemented with lactose. Additionally, resistance against salmonellae cecal colonization was further enhanced in treatment groups provided dietary lactose in combination with an undefined mixture of anaerobic bacteria (i.e., undefined competitive exclusion culture or Nurmi culture) originally obtained from the ceca of adult broiler chickens maintained on a diet containing lactose. In order to make a defined competitive exclusion culture that was efficacious in enhancing colonization resistance against salmonellae, we cultured cecal contents obtained from a 6-week-old pig maintained on a unmedicated diet in a continuous-flow (CF) culture apparatus (i.e., chemostat), that was maintained at parameters that would best represent the cecal environment. This research is being conducted under a CRADA with our industry partners and in cooperation with the USDA/ARS Salmonellosis in Swine research unit at Ames, Iowa. Presently a CF- culture has been developed and is being used in laboratory experiments to determine if this type of pathogen intervention strategy can also be used in the swine industry to aid in the reduction of enteric pathogen colonization in the swine gut.