Skip to main content
ARS Home » Research » Publications at this Location » Publication #73443
Title: HPLC METHODS TO ISOLATE PEPTIDE NEUROTRANSMITTERS

Author
item Holman, G
item HAYES, TIMOTHY - TEXAS A&M UNIVERSITY

Submitted to: Book Chapter
Publication Type: Book / Chapter
Publication Acceptance Date: 9/30/1996
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Biologically active peptides have become an increasingly active area of research in virtually all of the biomedical/biological sciences. This chapter describes, in a stepwise fashion, a tried and true method for the extraction, isolation, and purification of peptides from sources ranging from whole insects to dissected organs of any animal. Included are many tips and procedures to reduce or eliminate losses of peptides during purification such as sample stabilization, column loading, prevention of adsorption to plastics, and when to discard columns. The procedures are listed in such a way that a novice investigator can understand and follow the procedure with an excellent chance of successfully purifying the peptide of interest.