Submitted to: Congress on In Vitro Biology
Publication Type: Abstract Only
Publication Acceptance Date: 3/1/1996
Publication Date: N/A
Technical Abstract: A PCR assay was conducted to determine the presence of the mariner transposable element in established insect cell lines using primers corresponding to amino acids conserved between the mariner transposons of Drosophila mauritana and a moth, Hyalophora cecropia. Of the 17 insect cell lines examined, the mariner was detected in 12 cell lines. Southern hybridization using a digoxigenin-11-dUTP labeled random primed DNA probe of the mariner will be used to confirm whether the PCR products obtained from each of the cell lines are putative mariner elements. This may have important implications in the development of transformation vectors for use in insect cell lines.