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Title: INDUCTION OF GLYCOPROTEIN B (GB)-SPECIFIC CYTOTOXIC T CELLS AFTER VACCINATION WITH RECOMBINANT FOWLPOX VIRUS EXPRESSING GB

Author
item SCHAT, K - CORNELL UNIVERSITY NY
item OMAR, A - CORNELL UNIVERSITY NY
item Lee, Lucy
item Hunt, Henry

Submitted to: International Marek's Disease Symposium Abstracts and Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 9/7/1996
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Nazerian et al. (J. Virol. 66:1409, 1992) reported that chickens vaccinated with recombinant fowlpox virus expressing Marek's disease virus (MDV) gB (rFPV0-gB) were protected against challenge with MDV. Virus-neutralizing antibodies were detected in these chickens, but the presence of gB-specific cytotoxic T cells (CTL) was not examined. We investigated the possible role of CTLs in protective immunity after vaccination with rFPV-gB. Groups of P2a (B**19 B**19) chickens were vaccinated with SB-1, rFPV-gB or FPV were left uninfected. CTLs were obtained from spleens between 5 and 14 days post inoculation (dpi) and used in chromium release assays against reticuloendotheliosis virus-transformed cell lines expressing MDV gB or pp38. CTLs specific for pp38 and gB were present in SB-1-vaccinated chickens between 5 and 14 dpi, while gB-specific CTL were present in rFPV-gB inoculated birds between 7 and 14 dpi.