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Title: NUCLEOTIDE SEQUENCE ANALYSIS OF A 9-KB REGION OF MAREK'S DISEASE VIRUS GENOME EXHIBITS A COLLINEAR GENE ARRANGEMENT WITH UL30 TO UL34 GENES OF HERPES SIMPLEX VIRUS

Author
item WU, PING - MICHIGAN STATE UNIVERSITY
item SUI, DEXIN - MICHIGAN STATE UNIVERSITY
item Lee, Lucy

Submitted to: International Marek's Disease Symposium Abstracts and Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 9/7/1996
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: We reported the nucleotide sequence of the 9-kb BamHI fragment E of serotype 1 GA strain of Marek's disease virus (MDV) genome. DNA sequence analysis revealed five complete open reading frames (ORFs). Each of these ORFs exhibited strong amino acid sequence homology to the gene products of alpha-herpesviruses, and were arranged collinearly with the prototype sequence of HSV in the range of the UL30 to UL34 genes. Some of the DNA fragments were cloned into pATH vectors to express the trpE fusion proteins. Antisera were developed in rabbits against trpE fusion proteins: UL30, UL32, UL33, and UL34. The expressions of these genes in the chicken embryo fibroblast cells infected with MDV GA strain were confirmed by immunoprecipitation or Northern hybridization analysis. The potential function of these gene products will be discussed.