Submitted to: Annual Meeting and Expo of the American Oil Chemists' Society
Publication Type: Abstract Only
Publication Acceptance Date: 4/28/1996
Publication Date: N/A
Technical Abstract: High Performance Liquid Chromatography (HPLC) with a cyano phase column and a gradient mobile phase of methyl tert-butyl ether and hexane and a flame ionization (FID) detector was used to separate and quantitate mono, di and triglycerides, free fatty acids and methyl esters. These components can occur together during glycerolysis, lipolysis, randomization and interesterification reactions of vegetable oils like soybean. It is important for evaluation of those reactions to know both the identity and accurate quantity of the reaction products. Previously, we found the FID gave good accuracy without response factors for quantitative analysis by reversed phase HPLC of triglycerides. We evaluated in this work the response of the FID for HPLC of mixtures which contained other molecular species in addition to triglycerides. Gravimetric standards were evaluated by HPLC-FID in which each contained mono, di, and triglycerides, free fatty acids and methyl esters of palmitic, stearic, oleic, linoleic and linolenic acids repectively. The resolution of these components was better accomplished on a polar or cyano phase instead of a nonpolar or hydrocarbon phase HPLC column. Good accuracy was obtained by HPLC-FID for soybean glycerolysis products, which contained mono, di and triglycerides.