Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: 7/27/1996
Publication Date: N/A
Technical Abstract: A semi-selective medium was developed to induce blue pigmentation for detection of F. solani from infested soils and infected soybeans. A basal medium was used with different carbohydrates at different concentrations to induce blue pigmentation using a pure culture of the fungus. Of the carbohydrates tested, sucrose at 20 g/l produced the most consistent blue pigmentation. With the addition of quintozen and four antibiotics, the semi-selective medium was compared to Nash's medium using F. solani-infested soil with a range of 102 to 106 conidia/g added to pasteurized soil. The recovery rate from each medium was similar; however, the semi-selective medium induced blue colonies while Nash's medium did not. The semi-selective medium has been used to detect the fungus from infected roots and from field soil.