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ARS Home » Research » Publications at this Location » Publication #69266
Title: CHARACTERIZATION OF FUNGAL SPORES OF PHIALOPHORA GREGATA BY FLOW CYTOMETRY

Author
item GOURMET, CATHERINE - UNIV OF ILLINOIS
item Gray, Lynn
item RAYBURN, ALBERT - UNIV OF ILLINOIS

Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: 7/21/1996
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Flow cytometry was used to characterize isolates of Phialophora gretata using the fluorescence intensity of propidium iodide-stained conidia. Isolates differed in their mean fluorescence intensity, ranging from 89.4 to 116.0. When the number of fluorescent events was plotted against intensity of fluorescence, a single peak was observed. Fluorescent patterns of Phialophora-like fungi isolated from soybean vascular tissue were compared to isolates of P. gregata. Their mean fluorescence intensity ranged from 51.5 to 66.8. Multiple peak histograms were observed corresponding to multiple spore sizes as well as single and double nucleated conidia. Using flow cytometry, we were able to distinguish P. gregata isolates from Phialophora-like fungi isolated from soybean vascular tissue, based on mean fluorescence intensity and/or the presence of multiple peaks.