|Guill, Katherine - Kate|
|Coe Jr, Edward|
Submitted to: Maize Genetics Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 3/14/1996
Publication Date: N/A
Citation: N/A Interpretive Summary:
Technical Abstract: The advent of molecular techniques, including cloning and RFLP mapping, has provided the opportunity to begin to merge classical phenotypic mapping information with information about functionality. A group of almost 100 phenotypic mutants have been placed on chromosome 1 relative to RFLP markers. The mutants have been previously located to arm using translocation stocks. Mutants were mapped relative to the UMC Core Marker Set and bin locations assigned. The same Core Markers were used in parallel to produce an RFLP map containing 170 markers on chromosome 1 with a high preponderance of cDNAs or genomic clones of known function. Using the commonality of the Core Markers, we were able to define a genetic map containing both cDNAs and mutants on the same framework. The results indicate that this approach can: 1) identify potential cDNA/mutant associations, 2) indicate possible allelic pairs of mutants, and 3) provide rapid and cost-effective means of gene localization.