|JURTSHUK, JR., P|
Submitted to: American Society of Microbiologists Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 5/23/1996
Publication Date: N/A
Technical Abstract: Phenotypic and small subunit rRNA analysis were performed on nine marine Bacillus isolates. Forty-three different biochemical characteristics were included for phenotypic description including maximum and minimum growth temperatures and colony pigmentation. Genomic DNA was isolated from the isolates and PCR reactions were used to amplify the 16S rDNA genes which were sequenced directly using an Applied Biosystems Automated DNA Sequencer. Sequence analysis was performed on 1100+ bases of each isolate and compared with a data base of low G+C gram positive organisms. Four of the isolates can be tentatively identified as closely related but not identical to B. subtilis, B. pumulis, B. megaterium and B. sphaericus. The five remaining strains appear to be distinct species, with at least one isolate, an unusually thin rod forming very small spores, as a possible separate genus within the family Bacilliaceae.