Submitted to: Journal of Animal Science Supplement
Publication Type: Abstract Only
Publication Acceptance Date: 6/10/1996
Publication Date: N/A
Technical Abstract: The key role of chemokines (cytokines) in the orchestration of host defense, inflammatory, and other immune responses, prompted us to begin mapping these proteins as well as their receptors as part of a comprehensive program to examine their role in disease resistance. Restriction fragment length polymorphisms for four chemokine protein genes, transforming growth factor beta 2 and 3, interleukin 8 and monocyte chemoattractant protein 2 were developed by Southern blot hybridization after digestion of porcine genomic DNA with BamHI and PvuII, HindIII, BglII and PstI. Sufficient informative meiosis, 38, 28, 58 and 156, were available for us to pursue two-point pairwise linkage analysis of the four markers with over 1,000 existing loci in the USDA-MARC genome database to establish initial linkage (LOD > 3). Multi-point analysis determined the most likely order for each new marker. Chemokines TGFB-2, TGFB-3, IL-8, and MCP-2 were mapped to linkage groups on chromosomes 10p, 7q, 8q and 12q respectively. The human homologues of TGFB-2, TGFB-3, and IL-8 are on chromosomes 1p13, 14q13 and 4q24 respectively and the MCPs are clustered on human chromosome 17q. The assignment of TGFB-2, TGFB-3, IL8 and MCP-2 genes in swine concurs with previous porcine/human chromosomal homologies based on results from Zoo-FISH experiments. These findings add four new informative type I markers within a single gene family to the swine genome.