Submitted to: Federation of American Societies for Experimental Biology Conference
Publication Type: Proceedings
Publication Acceptance Date: 3/20/1996
Publication Date: N/A
Technical Abstract: Gastrointestinal parasites, especially Ostertagia ostertagi (Oo) are responsible for considerable economic losses in cattle. We have begun to characterize the local immune responses against Oo in the course of a primary infection. A method was developed to obtain viable intraepithelial (IEL) and lamina propria lymphocytes (LPL) from the ruminant abomasum. The recovery of LP cells was accomplished by enzymatic digestion, using dispase. Further purification of lymphocytes was performed by discontinuous percoll gradients. The cell surface phenotype of LPL and abomasal lymph node (ABLN) lymphocytes from control and Oo infected animals were compared by indirect immunofluorescence staining. Within 3 weeks of infection, the number of lymphocytes recovered from the gastric lamina propria was greatly increased. Increased levels of immunoglobulin-bearing cells, gamma-delta T cells and activated T cells in both LP and ABLN were found in the infected animals. Initial results indicated that LPL and IEL show reduced proliferation to mitogens and to polyclonal activators. The strongest proliferation was observed after stimulation with Con A. Cytokine gene expression for bovine IL2, IL4, IL10 and gamma interferon was detected in both IEL and LPL, but the level of mRNAs for IL2 and IL4 were very low. Study supported by a grant from INIA (Spain).