Submitted to: International Symposium on Selenium in Biology and Medicine
Publication Type: Abstract only
Publication Acceptance Date: 2/5/1996
Publication Date: N/A
Citation: Interpretive Summary:
Technical Abstract: Men and women living in an area of low Se intake were screened for plasma Se concentration and red cell glutathione peroxidase (GSH-Px) activity. Fifty subjects, with the lowest measures of Se and GSH-Px, were admitted into the study. Prior to the beginning of the supplementation portion of the study, each subject was given an oral dose of 74Se, and blood was collected at 0, 4, 8, 24, 48, 168 and 336 hours. Platelets, plasma, and red cells were prepared, digested and analyzed for 74Se by mass spectrometry. After the completion of this phase, subjects were randomly divided into 5 groups, and given either a placebo, or supplemental selenomethionine containing 10, 20, 30 or 40 ug Se/day. Supplementation was continued for six months, and during the last three weeks of supplementation, the stable isotope experiment was repeated as before. Maximal retention of the isotope in plasma was after 8 h [0.0081+/-0.0011 and 0.0071+/-0.0012 % (mean +/- SD) total dose/mL plasma, 1st and 2nd dose, respectively]. Average retention of the isotope in plasma was almost always lower after the 2nd dose, and retention of the 2nd dose tended to be negatively correlated with the amount of daily supplement. Maximal isotopic retention by platelets tended to be after 168 h. Retention after 168 h tended to be lower with increasing amount of supplement. These data show that supplemental Se, given to people with low Se status, alters the retention of a dose of stable Se.