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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Food Safety and Enteric Pathogens Research » Research » Publications at this Location » Publication #66325
Title: SALMONELLA IN GROWER/FINISHER SWINE: A SURVEY

Author
item CRAY, PAULA - 3630-14-00
item BUSH, E - USDA-APHIS-VS-CEAH
item THOMAS, L - USDA-FSIS-NVSL
item GRAY, J - 3630-14-00

Submitted to: American Society for Microbiology
Publication Type: Abstract Only
Publication Acceptance Date: 5/23/1996
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: The USDA:APHIS:VS conducted a pilot study of Salmonella spp. in swine herds as part of the NAHMS Swine 95 survey. Fifty fresh fecal samples (25 g/sample) were collected from finisher age pigs from 15 herds. One g of feces from each sample was placed into each of two culture media; tetrathionate broth (Tet) or GN Hajna broth (GN) followed by subculture into Rappaport R-10 medium (GN-R and Tet-R). All Tet, GN-R, and Tet-R wer struck onto brilliant green agar with sulfadiazine (BGS); Tet was also struck onto xylose-lysine-Tergitol 4 (XLT4) and brilliant green agar with novobiocin (BGN) plates. The sample and herd prevalence rates were 3.8% (27/715 positive) and 40% (6/15 positive), respectively. The predominant serotype identified was S. derby (93%; 25/27). Salmonella spp. were most often recovered from samples cultured in Tet-R (93%). Culture in GN-R was least sensitive. Identification of positive herds was best achieved by culture in Tet-R (83.3%) when compared to either Tet alone (50%) or GN-R (33.3%). Additionally, culture in Tet-R was most likely to identify all positive samples within a herd. No differences between plating media (BGS, BGN, and XLT4) were observed. These data provide information on the status of Salmonella spp. from finisher age swine and serve as baseline information for the larger Swine 95 survey. Additionally, these data demonstrate that Salmonella spp. has a higher probability of being recovered following use of both Tet and Rappaport R-10 media than any other medium.