Author
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Hunt, Curtiss |
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VANDERPOOL, RICHARD - 5450-10-00 |
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Submitted to: Journal of Federation of American Societies for Experimental Biology
Publication Type: Abstract Only Publication Acceptance Date: 4/14/1996 Publication Date: N/A Citation: N/A Interpretive Summary: Technical Abstract: Identification of specific boron-dependent metabolic processes in animals may be expedited by a better understanding of boron kinetics. Weanling male rats (36 per group) were fed a ground corn, high protein casein, and corn oil-based diet (approx. 0.5 g B/kg) supplemented with boron at 0 or 1.2 mg/kg for 68 d. After a 16 h fast, 24 rats from each dietary group were fed a test meal (TM) containing natural abundance (NA)B or 10**B intrinsically-labelled broccoli. Rats were killed between 0.7 and 3.2 hr after the TM. Blood plasma, washed erythrocytes, whole brain, heart, and spleen, and liver slices were collected. Boron concentrations and isotope ratios were determined by inductively coupled plasma-mass spectrometry and expressed as % dose = (nmole 10**B>NA/g or ml wet sample/[314 nmole 10**B>NA/g TM)(g TM fed)(100)] with corrections for blood boron as appropriate. Gut contents accounted for over 60% of the 10**B dose at the end of the measured time period, a finding that indicates boron absorption from natural foods, compared to inorganic sources as reported earlier, occurs at a slower rate. In rats fed the 10**B-enriched TM, the %dose increased over the measured time period in brain, spleen, liver, plasma, and decreased in heart. This finding suggests that boron turnover is relatively fast in body tissues or conversely, that boron repletion of body tissue pools is relatively rapid. |
