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ARS Home » Plains Area » Grand Forks, North Dakota » Grand Forks Human Nutrition Research Center » Dietary Prevention of Obesity-related Disease Research » Research » Publications at this Location » Publication #65762
Title: FATTY ACIDS AFFECT IRON UPTAKE AND TRANSPORT IN CACO-2 CELLS

Author
item DROKE, ELIZABETH - 5450-20
item JOHNSON, LUANN - UNIV OF NORTH DAKOTA
item Lukaski, Henry

Submitted to: Journal of Federation of American Societies for Experimental Biology
Publication Type: Abstract Only
Publication Acceptance Date: 4/14/1996
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Dietary fat can alter iron (Fe) absorption and utilization, intestinal cell Fe and aconitase activity in rats. The Caco-2 cell line was used to determine the effect of fatty acids (FA) on the cellular acquisition (uptake) and transport of 59Fe in the human intestine. Cells were cultured in medium supplemented with 1 or 10% fetal calf serum (FCS; BDM1 or BDM10). Fatty acids (stearic acid, SA; palmitic acid, PA; linoleic acid, LA; oleic acid, OA) were complexed to bovine serum albumin (BSA) for supplementation; BSA was included as a control. In BDM1 cells, uptake of 59Fe at 5 h was affected (P<0.05) by FA; BSA < LA < SA < PA < OA. Transport (5 min to 5 h) of 59Fe from the apical to basal compartment was lowest (P<0.05) with BSA and LA, and increased (P<0.05) with SA, OA and PA. In BDM10 cells, uptake at 5 h was lowest (P<0.05) with BSA and SA, and increased (P<0.05) with PA and OA; cells receiving LA had uptake values intermediate between BSA and SA, and PA and OA. Transport in BDM10 cells also was affected (P<0.05) by FA; BSA < SA, LA < PA, OA. Transport of 59Fe was reduced (P<0.05) by inclusion of 1 (BDM1) or 10% (BDM10) FCS in the basal compartment medium when compared to BSA (BDM-50uM BSA). These data suggest that the type of fatty acid can affect iron uptake and transport in the human intestine.