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ARS Home » Research » Publications at this Location » Publication #65698
Title: FUNGAL ENZYMES ASSOCIATED WITH SELECTED VINE DECLINE PATHOGENS OF CANTALOUPE

Author
item Bruton, Benny
item BILES, C - EAST CENTRAL UNIVERSITY
item CLUCK, T - EAST CENTRAL UNIVERSITY
item GARCIA-JIMENEZ, J - UNIVERSITY POLITECNICA

Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: 2/3/1996
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Macrophomina phaseolina, Didymella bryoniae, Phomopsis cucurbitae, Monosporascus cannonballus and, an Acremonium sp. are capable of causing vine declines. However, M. cannonballus (Spain) and Acremonium sp. (Spain) are the only two fungi that do not also cause a fruit rot. The cell wall degrading enzymes produced by these pathogens on cantaloupe plant tissue were determined. Enzymes assayed were beta-galactosidase (BG), alpha-mannosidase (AM), polygalacturonase (PG), and cellulase (CE). The fungi were grown in a minimal salt broth with either commercial pectin, CM-cellulose, cantaloupe root, hypocotyl or stem tissue as the substrate. Aliquots were removed from the shake cultures after 0,3,6, and 9 days of growth. Bioassays indicated that AM was not produced by any of the fungi on the substrates described. BG, CE, and PG were produced by M. phaseolina, D. bryoniae, and P. cucurbitae. Acremonium sp. produced CE and PG, whereas M. cannonballus produced CE only.