Submitted to: Society of Nematology Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 10/15/1995
Publication Date: N/A
Technical Abstract: Columns (38-mm-d, 40 or 72 mm long) of sand (94% less than 250-um particle size) wetted with a synthetic soil solution (10-12% moisture) were used to examine responses of second-stage juveniles (J2) of Meloidogyne incognita race 3 to calcium alginate pellets of Monacrosporium cionopagum, M. ellipsosporum, and Hirsutella rhossiliensis. A layer of 10 or 20 pellets was placed 4 or 20 mm from one end of the column. After 0, 3, or 14 days, J2 were put on both ends, on one end, or in the center. Second-stage juveniles were extracted from 8-mm sections 1 or 2 days later. All three fungal pellets were repellent; pellets without fungi were not. Aqueous extracts of pellets and of sand in which pellets had been incubated were repellent, but acetone extracts redissolved in water were not. Injection of CO2 (20 ul/minute) into the pellet layer attracted J2 and increased mortality. However, parasitism of M. javanica J2 by all three pelletized fungi, and invasion of cabbage seedling roots in vials containing four pellets and 17-cm3 of loamy sand indicated repellency had little effect on efficacy.