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Title: IMPROVED COSMID VECTORS FOR LIBRARY CONSTRUCTION, SYSTEMATIC CHROMOSOME WALKING AND RAPID RESTRICTION MAPPING IN USTILAGO MAYDIS AND MAGNAPORTHE GRISEA

Author
item AN, ZHIQIANG - UNIVERSITY OF WISCONSIN
item FARMAN, MARK - UNIVERSITY OF WISCONSIN
item Budde, Allen
item TAURA, SATORU - UNIVERSITY OF WISCONSIN
item Leong, Sally

Submitted to: Gene
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/26/1996
Publication Date: N/A
Citation: N/A

Interpretive Summary: Transformation is an important tool of molecular biology. This technique allows the introduction of foreign DNA into an organism. We are studying the molecular biology of pathogenesis of the rice blast fungus Magnaporthe grisea and the corn smut fungus Ustilago maydis. We have previously established methods of transformation for this fungi. In this report we describe new plasmid cloning tools for these organisms.

Technical Abstract: New cosmid vectors were constructed for the ascomycete fungus Magnaporthe grisea and basidiomycete fungus Ustilago maydis. These vectors are capable of transforming M. grisea at frequencies of up to 5 transformants/ug linear DNA and U. maydis at frequencies of up to 25 transformants/ug DNA when using an autonomously-replicating vector. Since the promoters used in these vectors function in other-asco- and basidiomycete fungi, we anticipate that these vectors will be widely applicable.