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Title: USE OF RAPD AND SPECIES-SPECIFIC PCR PRIMERS AS AIDES IN IDENTIFYING SDS ISOLATES OF FUSARIUM SOLANI

Author
item Gray, Lynn
item ACHENBACH, LAURIE - SOUTHERN ILLINOIS UNIV

Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: 10/1/1995
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Randomly amplified polymorphic DNA (RAPD) markers were used to characterize different isolates of Fusarium solani that cause soybean sudden death syndrome. Twenty isolates representing collections from various locations in Illinois and other states were evaluated along with eight isolates of F. solani from other plant hosts. The SDS isolates were readily distinguished from other non-SDS isolates based on banding patterns generated with five 10-base primers. Repeatability of banding patterns with different primers has been compared between laboratories using different sources of Taq DNA polymerase, DNA samples, and thermocyclers. A species-specific PCR primer pair was used to separate SDS isolates from non-SDS isolates of F. solani. This primer pair specifically amplifies DNA from only SDS isolates and can be used to identify newly collected isolates. Pathogenicity tests of all fungal isolates are being conducted in the greenhouse to determine the severity of root rot on inoculated soybean plants as well as the severity of foliar SDS symptoms.