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Title: RFLP AND RAPD ANALYSES OF SOLANUM BULBOCASTANUM DNA IN PROGENY OF A SOMATICHYBRID WITH POTATO

Author
item MCGRATH, MITCHELL - UNIVERSITY OF WISCONSIN
item WEILGUS, SUSAN - UNIVERSITY OF WISCONSIN
item Haberlach, Geraldine
item Helgeson, John

Submitted to: Potato Association of America Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 7/24/1995
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: We are continuing our analyses of somatic hybrids and their progeny of potato combined with various sexually-incompatible Solanum species for their potential to contribute valuable disease resistances. Our previous work has focused primarily on somatic hybrids of potato and S. brevidens. Using molecular markers to follow S. brevidens chromosomes, we have demonstrated that S. brevidens chromosomes can be transmitted through at least the fourth backcross generation. Also, some S. brevidens chromosomes can substitute for S. tuberosum chromosomes and S. brevidens chromosomes recombine non-homologously with other S. brevidens chromosomes. Finally, it is clear that introgression of S. brevidens chromosome-specific markers occurs. These observations suggest the potato and S. brevidens genomes are not highly diverged, despite barrier(s) to sexual hybridization. In a similar manner, we are beginning to examine progeny of S. bulbocastanum + potato somatic hybrids with molecular markers for evidence of recombination and introgression. To date, 12 S. bulbocastanum synteny groups have been identified with 151 RAPD markers in one BC2 mapping population and recombinants have been identified for each of these 12 chromosomes.