Submitted to: Phytopathology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 12/3/1992
Publication Date: N/A
Citation: Interpretive Summary: A new virus, Peanut Chlorotic Ringspot Virus, was discovered in Stillwater, OK. This virus causes wide bright chlorotic rings on plant leaves, symptoms unlike those of any previously reported peanut viruses in the United States. Detailed evaluations were conducted to characterize the virus. The discovery of this virus is important from both academic and applied points of view and will be used by peanut researchers and the peanut industry.
Technical Abstract: A virus causing wide chlorotic ringspot (PCRV) associated with chlorotic line pattern and mottling was discovered on an Erictoides hybrid growing in USDA-OSU greenhouses, Stillwater, OK. The virus was isolated, characterized, and found to differ in symptomology, host range and serological properties from all previously described viruses infecting peanut, particularly those reported in the US to be the most important ones. The virus was transmitted by both mechanical inoculation and grafting to 14 peanut cultivars, causing identical symptoms as originally observed on the Erictoides hybrid. In addition to peanut, the virus systemically infected Pisum sativum L. 'Little marvel' causing mainly mosaic and Lupinus albus L. 'Tiftwhite' producing severe malformation and remarkable reduction in leaflet area. The virus did not infect many other plant species known to be susceptible hosts to peanut mottle virus. Phaseolus vulgaris L. 'Topcrop' and Chenopodium amaranticolor Coste & Reyn were found to be two useful local lesion assay and diagnostic hosts for PCRV. The virus elicited necrotic local lesions on the first and chlorotic ringspots on the second. PCRV had a dilution end point between 10**-5 and 10**-6, thermal inactivation point between 55 and 60 deg C, and longevity in vitro up to 6 but not 7 days. Virus particles viewed by electron microscopy and the negative stain uranyl acetate were flexuous filamentous particles ranging in length from 750 to 850 nm. In both indirect PAS-ELISA and Ouchterlony double immunodiffusion test, PCRV was serologically related to a PMV isolate from Oklahoma (PMV-OK) but not to bean yellow mosaic virus, peanut stripe virus, potato virus Y, watermelon mosaic virus 1, watermelon mosaic virus 2, wheat streak, and zucchini yellow mosaic virus.