Skip to main content
ARS Home » Midwest Area » St. Paul, Minnesota » Plant Science Research » Research » Publications at this Location » Publication #60848
Title: ASSOCIATION OF OAT RFLP LINKAGE GROUPS WITH CHROMOSOMES USING TRUE AND IMMORTAL F1 MONOSOMIC SERIES

Author
item FOX, STEPHEN - UNIVERSITY OF MINNESOTA
item Rines, Howard
item PHILLIPS, RONALD - UNIVERSITY OF MINNESOTA

Submitted to: Stadler Genetics Symposium
Publication Type: Abstract Only
Publication Acceptance Date: 6/20/1995
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: A genomic linkage map of oat (Avena sativa L.) constructed with more than 500 RFLP markers in a Kanota/Ogle cross contains an excess number of linkage groups compared to the expected 21, one for each chromosome of oat. Aneuploids are being used to consolidate the map and to associate markers to physical chromosomes. Utilizing the Kanota oat monosomic series in crosses with Ogle and the Sun II oat monosomic series in crosses with Ogle and Kanota, DNA preparations from both monosomic F1 plants and bulked F2 plants used to mimic the F1 were screened with probes obtained from the Kanota/Ogle oat RFLP map. Positive associations between probe binding sites and chromosomes were made only when the paternal form of an RFLP marker was present and the maternal form was absent. Oat map linkage groups could be associated with specific chromosomes using this method. Genetically unlinked loci were shown to be physically associated on the same chromosome. Heterogeneity and duplications among lines within the monosomic series have been detected.