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ARS Home » Midwest Area » St. Paul, Minnesota » Plant Science Research » Research » Publications at this Location » Publication #60119
Title: DEVELOPMENT AND APPLICATIONS OF TRANSFORMATION FOR OAT IMPROVEMENT

Author
item SOMERS, DAVID - UNIVERSITY OF MINNESOTA
item TORBERT, KIMBERLY - UNIVERSITY OF MINNESOTA
item MILACH, SANDRA - UNIVERSITY OF MINNESOTA
item PAWLOWSKI, WOJCIECH - UNIVERSITY OF MINNESOTA
item Rines, Howard

Submitted to: Value Added Cereals Through Biotechnology Conference
Publication Type: Abstract Only
Publication Acceptance Date: 6/13/1995
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Oat transformation was first reported in 1992 based on microprojectile bombardment of friable, embryogenic callus. Several improvements have recently been made in the transformation system that have: 1. enabled the use of selectable marker genes that pose reduced ecological risk; 2. contributed to increased frequencies of regeneration of fertile, transgenic cplants; and 3. allowed initiation of experiments to apply the transformation system to oat improvement. Molecular and genetic analyses of transgene integration and inheritance have been conducted to evaluate stability of transgene inheritance and expression. Applications of the oat transformation system involve investigation of mechanisms for resistance to barley yellow dwarf virus, powdery mildew, and stem rust. We also have conducted an expressioanalysis f of the commelina yellow mottle virus promoter. Progress in these projects will be reported.