Submitted to: In Vitro Haploid Production in Higher Plants
Publication Type: Book / Chapter
Publication Acceptance Date: 7/11/1995
Publication Date: N/A
Citation: Interpretive Summary:
Technical Abstract: Haploid plant recovery by anther culture in hexaploid oat (Avena sativa L.) has been infrequent with only two reports of successful plant recovery. In the first report only three plants were recovered, all from a single anther. The other report described the recovery of a total of twelve green plants. Callus initiation has been frequently observed, especially on hormone free initiation media, but identification of conditions that would promote subsequent plant regeneration has been elusive. Wide cross oat x maize hybridizations involving maize chromosome elimination during early embryonic cell divisions with subsequent embryo rescue has provided an alternative source of oat haploids. Haploid oat plants have the unique property of being partially self fertile through a process of meiotic restitution. This self fertility yields both euploid and aneuploid progeny. Another unique feature we discovered is that maize chromosome elimination is not always complete in oat x maize hybrid embryo development. Haploid oat plants retaining up to four maize chromosomes have been identified. Ten plants with one, and one plant with two, maize chromosomes were self fertile. Using maize molecular marker probes, disomic maize chromosome addition lines of oat (2n = 42 + 2) were identified that carried maize chromosomes 2, 3, 4, 7, 8, and 9 and a double disomic maize chromosome addition oat line (2n = 42 + 4) was identified that carried maize chromosomes 4 and 7. The novel properties of partial self fertility in haploid oat and the retention of maize chromosomes in fertile derivatives of oat x maize crosses provide exciting materials for gene transfer and mapping analyses.