Author
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Akin, Danny |
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Submitted to: Southern Pasture and Forage Crop Improvement Conference Proceedings
Publication Type: Proceedings Publication Acceptance Date: 5/15/1995 Publication Date: N/A Citation: N/A Interpretive Summary: An interpretive summary is not required for a literature review, book chapter (unless reporting original research), book review, or oral presentation (where only the title or abstract is published. Refer to directive 152.1 4/3/91 Technical Abstract: Histochemistry and UV absorption microspectrophotometry are useful to identify the location of aromatic constituents within walls of forage cell types. Histochemical stains can differentiate the types of aromatics to some extent, but characterization of the aromatic constituent is not absolute and compounds are not quantitated. Generally, AP designates the most recalcitrant cell types to biodegradation, while those designated by CS are less resistant and at times partially digestible. Results from grass cell walls indicate that ester-linked phenolic acids, rather than syringyl groups of polymerized aromatics, likely are responsible for CS+ reactions in walls of cell types such as parenchyma bundle sheaths in leaf blades and mature stem parenchyma. Diazonium salts have a known chemical reaction with phenolics and offer a more definitive chemical proof of the presence of these constituents in cell walls. These stains are often quite ehelpful in delineating the presence of aromatic compounds in walls of slowly digested but non-lignified, living cells such as parenchyma bundle sheaths of leaf blades in warm-season grasses. |
