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ARS Home » Midwest Area » St. Paul, Minnesota » Plant Science Research » Research » Publications at this Location » Publication #59531
Title: GENETIC TRANSFORMATION IN AVENA SATIVA L. (OAT)

Author
item SOMERS, DAVID - UNIVERSITY OF MINNESOTA
item Rines, Howard
item TORBERT, KIMBERLY - UNIVERSITY OF MINNESOTA
item PAWLOWSKI, WOJIECH - UNIVERSITY OF MINNESOTA
item MILACH, SANDRA - UNIVERSITY OF MINNESOTA

Submitted to: Biotechnology in Agriculture and Forestry
Publication Type: Book / Chapter
Publication Acceptance Date: 6/9/1995
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Genetic transformation of cultivated hexaploid oat (Avena sativa L.) was first reported in 1992. Several improvements have been incorporated into the transformation system since that time. The current system is based on microprojectile bombardment of friable, embryogenic callus with plasmids carrying the Escherichia coli gene npt II. The incorporation and expression of this gene in oat cells allows survival and selection on a medium containing the antibiotic paromomycin. About 0.6 transgenic tissue cultures that regenerated fertile plants are produced per microprojectile bombardment treatment. More than half of the transgenic oat lines produced progeny whose transgene sequences and expression conformed to expected Mendelian segregation ratios for one or two genes. The oat transformation system, while productive, still requires improvements primarily because it is most efficient with a specific genotype that has poor agronomic characteristics. The oat transformation system will enable novel modifications for kernel quality, disease resistance, and other traits not feasible using conventional genetic manipulations