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ARS Home » Midwest Area » Columbia, Missouri » Plant Genetics Research » Research » Publications at this Location » Publication #59454
Title: THE EXPRESSION OF AL-STRESS-INDUCED POLYPEPTIDES IN A POPULATION SEGREGATING FOR AL-TOLERANCE IN WHEAT (TRITICUM AESTIVUM L.)

Author
item SOMERS, DARYL - UNIV OF MO
item Gustafson, J

Submitted to: Genome Research
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/31/1995
Publication Date: N/A
Citation: N/A

Interpretive Summary: Many stresses in plants induce changes in normal protein synthesis, and characterizing these changes can reveal how plants cope with stress, or the changes in protein synthesis may be used as biological indicators of tolerant and sensitive plants. The current literature describing aluminum(Al)-induced changes in protein synthesis in wheat is inconsistent and shows there are very limited changes in protein synthesis and that non of the changes in protein synthesis are directly associated with Al-tolerant and Al-sensitive types. We analyzed protein synthesis in a pair of nearly identical lines of wheat, with the exception that one was Al tolerant and the other was Al sensitive. Aluminum caused many changes in protein synthesis in Al-sensitive wheat and had very little influence on protein synthesis in Al-tolerant wheat. In addition, all of the changes in protein synthesis observed were inherited and were directly associated with hthe level of Al tolerance. We conclude that Al tolerance in wheat was strongly associated with the maintenance of normal protein synthesis and that Al-induced changes in protein synthesis were indicators of Al- sensitivity.

Technical Abstract: This study examined the changes in gene expression induced by aluminum (Al) stress in wheat root tips. Seedlings of Triticum aestivum L. cvs 'Katepwa' (Al sensitive), 'Maringa' (Al tolerant), and the near isoline 'Alikat' (Al tolerant; 'Katepwa'*3/'Maringa') were grown for 3 days in either 0 or 1 ug/mL Al and polypeptides were labeled with 35 Smethionine prior to separation by gel electrophoresis. There were a few polypeptides from whole-cell lysates that showed enhanced expression in all of the genotypes in 1 ug/mL Al and thus were not correlated with either Al tolerance or sensitivity. However, the whole cell lysate and microsomal polypeptide profiles also revealed numerous changes in gene expression in 'Katepwa' at 1 ug/mL Al; these co-segregated with only the Al-sensitive F2 bulks. The microsomal polypeptide profiles of the Al-tolerant lines 'Maringa' and 'Alikat' changed marginally in the presence of Al and these changes were also reflected in the Al-tolerant F2 bulks. The data showed there were many changes in gene expression that co-segregated with Al sensitivity and that Al tolerance in wheat may rely on constitutively expressed polypeptides.