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Title: MACROMOLECULAR EQUILIBRIA AND KINETICS: LINKED THERMODYNAMIC FUNCTIONS CHAPTER 6

Author
item RUSLING, JAMES
item Kumosinski, Thomas

Submitted to: Nonlinear Computer Modeling of Chemical and Biochemical Data
Publication Type: Book / Chapter
Publication Acceptance Date: 5/4/1995
Publication Date: N/A
Citation: N/A

Interpretive Summary: Book Chapter interpretive summary is not required.

Technical Abstract: The Concept of Wyman's theory of linked functions is presented in chapter 6 as well as how this theory is applied to the analysis of macromolecular data using nonlinear regression methods. After a general introduction, the overall equilibrium expression and detailed derivation of applicable equations is given. A tutorial to contrast the cooperative and non-cooperative equilibrium models is next discussed. Several applications (diffusion coefficients of micelles by using electrochemical probes, influence of salt on the solubility of proteins , enzyme kinetics - Michaelis-Menten model, the pH dependence of enzyme activity, influence of multivalent cations on kinetics of a Cell Wall Enzyme and enzyme inhibition) of the use of thermodynamic linkage in conjunction with nonlinear regression analysis are presented. Experimental data for these applications are analyzed using nonlinear regression methods. Their derived equilibrium parameters with appropriate errors are interpreted to provide a complete molecular basis for the reader.