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ARS Home » Midwest Area » Ames, Iowa » Corn Insects and Crop Genetics Research » Research » Publications at this Location » Publication #58456

Title: THE RF2 NUCLEAR RESTORER OF MALE-STERILE T CYTOPLASM ENCODES AN ALDEHYDE DEHYDROGENASE

Author
item CUI, XIANGQIN
item CHEN, WEIJUN
item Wise, Roger
item SCHNABLE, PATRICK

Submitted to: Maize Genetics Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 3/14/1995
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Rf2 is one of two complementary nuclear restoration genes of the male-steri T cytoplasm. Transposon tagged alleles of rf2 have been isolated previousl (Schnable and Wise, Genetics, 1994). A 3.4-kb Rf2 fragment containing the Mu1 element was cloned and verified via allelic cross-referencing experiments. A partial Rf2 cDNA obtained from a tassel library exhibits 70 0amino acid sequence homology to aldehyde dehydrogenases. This finding has led to the development of a model to explain the role of Rf2 in fertility restoration. An analysis of an Rf2 genomic clone from B73 has established the presence of at least five introns.