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ARS Home » Midwest Area » Ames, Iowa » Corn Insects and Crop Genetics Research » Research » Publications at this Location » Publication #58455

Title: MOLECULAR ISOLATION AND ANALYSIS OF NUCLEAR RESTORER GENES FOR THE MALE- STERILE T CYTOPLASM

Author
item SCHNABLE, PATRICK
item CUI, XIANGQIN
item CHEN, WEIJUN
item DILL, CARREN
item Wise, Roger

Submitted to: Maize Genetics Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 3/14/1995
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: The male sterility associated with T cytoplasm is due to the action of a unique mitochondrial gene, T-urf13. Restoration of pollen fertility in T cytoplasm requires the complementary action of dominant alleles (Rf1 and Rf of two nuclear-restorer genes. It is known that Rf1 has a marked effect on the transcription and/or translation of T-urf13. However, this Rf1-mediate ealteration of T-urf13 transcription or translation is not sufficient for fertility restoration, since Rf2 is essential for restoration, and there is no evidence that Rf2 effects expression of T-urf13. As a first step toward unravelling the molecular processes involved in cms restoration we have utilized transposon tagging to generate a collection of rf1 and rf2 mutants Using these mutants, we have cloned the rf2 locus and are undertaking an analysis of its function.