Submitted to: Pacific Basin Society Chemical International Congress Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 12/17/1995
Publication Date: N/A
Technical Abstract: Bitterness due to limonin in a variety of citrus juices is a major problem in the citrus industry worldwide. Although various treatments have been shown to reduce the limonin concentration in juice, the most promising approach is to use genetic engineering to alter the citrus genome to create citrus plants that produce fruit free from limonin accumulation. The technology for such a gene insertion into cultured citrus plant cells and regeneration of mature citrus trees from these single cells is well established. We have identified three specific target enzymes for creation of transgenic citrus fruit free from limonin bitterness: limonoate dehydrogenase, UDP-D-glucose transferase and nomilin deacetylesterase. Limonoate dehydrogenase and UDP-D-glucose transferase have been isolated from Arthrobacter globiformis and the albedo tissues of navel orange, respectively. The amino acid sequences of N-terminal parts of these enzymes have been determined and further work on the isolation and insertion of these genes into citrus cells is in progress.