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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Food Safety and Enteric Pathogens Research » Research » Publications at this Location » Publication #431383
Research Project: Analysis of Genetic Factors that Increase Foodborne Pathogen Fitness, Virulence, and Antimicrobial Resistance Transfer, to Identify Interventions against Salmonella and Campylobacter in Food Animals

Location: Food Safety and Enteric Pathogens Research

Title: Three complete Salmonella enterica serovar Enteritidis genomes from the United States with and without a gyra d87y substitution

Author
item BRADSHAWW II, DAVID - Oak Ridge Institute For Science And Education (ORISE)
item WEBB, HATTIE - Centers For Disease Control And Prevention (CDC) - United States
item TAGG, KAITLIN - Centers For Disease Control And Prevention (CDC) - United States
item KIM, JUSTIN - Minnesota Department Of Health
item HAAN, JISUN - Minnesota Department Of Health
item HWANG-HINZ, HAEJIN - Minnesota Department Of Health
item Anderson, Christopher
item Bearson, Bradley
item Bearson, Shawn

Submitted to: Microbiology Resource Announcements
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/16/2026
Publication Date: N/A
Citation: N/A

Interpretive Summary: Of the more than 2600 serotypes of Salmonella, Salmonella enterica serovar Enteritidis (Enteritidis) is one of the most common serotypes to cause human foodborne-related illness, and chicken products are a major source. An increase in the proportion of Enteritidis with a DNA mutation (D87Y) in a gene product (GyrA) that results in decreased susceptibility to ciprofloxacin (DSC) has recently been observed. Ciprofloxacin is a critical antibiotic for treatment of severe Salmonella-induced gastrointestinal illness in humans; thus, this mutation leads to less effective treatments. To investigate genetic differences between Enteritidis isolates with and without the GyrA mutation, complete genome sequencing was performed on the isolates of interest. This genetic information provides data for comparison of the genomes to screen for additional genomic changes associated with the GyrA mutation that may influence chicken prevalence and environmental persistence of DSC Enteritidis, thereby providing targets for detection and reduction strategies.

Technical Abstract: The prevalence of Salmonella enterica serovar Enteritidis encoding a GyrA D87Y substitution, associated with decreased susceptibility to ciprofloxacin, has increased since 2018 in the United States. Here, we provide three Salmonella Enteritidis reference genomes belonging to a SNP cluster of interest with (n=2) or without (n=1) the GyrA D87Y substitution.