Efficient collection of skin biopsies using the Tissue Sampling Unit® for subsequent cryopreservation and culture of fibroblasts

Authors: Purdy, Phillip; Redel, Bethany; Chen, Paula; Rahe, Ashley; Jivan, Aashi; Spiller, Scott

Submitted to: Methods and Protocols
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/24/2025
Publication Date: N/A
Citation: N/A

Interpretive Summary: Tissue samples are a rich source of genetic resources because they can be easily collected, frozen as part of a genebank collection, and eventually used for genetic research or cloning to rebuild animal populations. However, collection of tissues with ear notchers, scalpels, or biopsy punches can be difficult because the equipment and samples can be easily contaminated. Therefore, we explored the use of the Allflex Tissue Sampling Unit (TSU) system to determine if it could be adapted to genebanking needs. The TSU system was simple, efficient, and samples that were collected could easily be frozen, thawed, and used for tissue culture resulting in large quantities of viable cells for research or repopulation. Based on these results, the methods and the TSU system can be used for genebanking purposes, by research labs, and by stakeholders who need to collect high quality, viable samples.

Technical Abstract: Dermal tissue samples are a rich source of germplasm because they can be readily collected, frozen as part of a genebank collection, digested and cultured, and used for a variety of purposes such as genotyping or other forms of genetic research. Derived fibroblasts can also be used for somatic cell nuclear transfer, and the remaining cells can be frozen for future use. However, collection of tissues with ear notchers, scalpels, or biopsy punches can be problematic because tissue handling and the tool surfaces can contaminate the samples. Therefore, the modification of the Allflex Tissue Sampling Unit (TSU) system was explored to determine if the technology can empower rapid collection of clean samples that are easily identifiable and portable. Results indicate that the TSU system was efficient, and samples that were collected and processed for tissue culture resulted in successful derivations of fibroblasts from 7 of 11 animals. Thus, the TSU system appears to be a viable option for collecting and preserving dermal tissue for genebanking and other applications where simple, rapid collection of large quantities of samples is required.