Phenotypic data on seedling traits of hexaploid spring wheat panel evaluated under heat stress

Authors: GUDI, SANTOSH - North Dakota State University; SINGH, JATINDER - North Dakota State University; GILL, HARSIMARDEEP - South Dakota State University; SEHGAL, SUNISH - South Dakota State University; Faris, Justin; UPINDER, GILL - North Dakota State University; Gupta, Rajeev

Submitted to: Data in Brief
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/10/2025
Publication Date: 9/16/2025
Citation: Gudi, S., Singh, J., Gill, H.S., Sehgal, S., Faris, J.D., Upinder, G., Gupta, R. 2025. Phenotypic data on seedling traits of hexaploid spring wheat panel evaluated under heat stress. Data in Brief. https://doi.org/10.1016/j.dib.2025.112069.
DOI: https://doi.org/10.1016/j.dib.2025.112069

Interpretive Summary: Wheat production is constrained by several biotic and abiotic stresses. Heat stress is one of the major abiotic stress that negatively impacts wheat growth at various developmental stages. Understanding the genetic basis of heat stress tolerance can help in breeding resilient wheat cultivars with improved productivity. In this study we evaluated a diverse panel of spring wheat landraces and cultivars under heat stress which revealed large phenotypic and genetic variations. Genomic regions and candidate genes identified in this study hold promise for developing heat-resilient wheat cultivars through use of makers assisted breeding.

Technical Abstract: Heat stress is the major abiotic stress affecting wheat at various developmental stages including seedling and reproductive stage. Heat stress at early developmental stages affects the seed germination and seedling establishment, thereby reduces grain yield per unit area. To overcome the negative impact of heat stress, it is crucial to identify the source of heat tolerant germplasm lines and also introduce them in breeding program. In this study, we evaluated 216 global diversity panel of hexaploid spring accessions comprising landraces and cultivars under non-heat stress (23°C) and heat stress (36°C) treatments. Phenotypic data was collected after 13 days of heat stress on various seedling traits, including coleoptile length (CL; cm), shoot length (SL; cm), root length (RL; cm), tiller number (TN), shoot fresh weight (SFW; mg), and root fresh weight (RFW; mg). Heat stress negatively affected all the seedling traits with maximum effect on RL (85.6% reduction) and minimum effect on CL (15.44%). However, the RN was increased by 20% under heat stress. It was also noticed that the effect of heat stress was more on root traits (such as RL and RFW) as compared to shoot traits (such as SL and SFW). This suggests that compared to roots, shoots may have adaptive mechanisms such as transpiration cooling via stomatal regulation, to alleviate the negative impacts of heat stress. Moreover, the raw phenotypic data was subjected to mixed linear analysis to derive best linear unbiased estimates (BLUEs). BLUE values were further used to assess the intrinsic relationship among the seedling traits under non-heat stress (23°C) and heat stress (36°C) treatments. The dataset presented in this study serves a valuable source for identifying extremely tolerant lines for heat stress, which can be utilized in breeding program to develop heat resilient, high-yielding wheat cultivars. Moreover, this dataset helps in identifying potential genomic regions associated with improved heat stress tolerance, which can be incorporate in marker-assisted breeding of heat tolerant wheat varieties.